Skip to main content
. 2016 May 20;4(10):e12791. doi: 10.14814/phy2.12791

Figure 5.

Figure 5

Activation of Akt/mTOR signaling or CaMKII/HDAC4 signaling in SOL muscle of WT and Epac1KO in response to chronic CB infusion. (A–B) Activation of Akt/mTOR signaling was examined by measuring phosphorylated and total Akt (A) and S6K1 (B) in SOL prepared from WT and Epac1KO after chronic CB treatment for 3 weeks. Significant increases of phosphorylated Akt (Ser 476) and S6K1 (Thr 389) molecules were not observed in either WT or Epac1KO (P = NS vs. Control by Tukey's test, n = 6 each). (C–D) Phosphorylated and total CaMKII (C) and HDAC4 (D) in SOL of WT and Epac1KO after chronic CB infusion for 3 weeks. Significant increases of phosphorylated CaMKII (Thr 286) and HDAC4 (Ser 246) were not observed in either WT or Epac1KO (P = NS vs. Control by Tukey's test, n = 6). The amount of expression in WT treated with saline was taken as 100% in each determination and representative immnunoblotting results are shown for phosphorylated and total Akt, S6K1, CaMKII, and HDAC4