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. 2016 May 24;4(10):e12814. doi: 10.14814/phy2.12814

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© 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Divalent cations increase the AHP. (A) Close‐ups of the AP's AHPs before (black solid) and after the addition of cadmium or nickel for 15 min at 35°C (gray dashed). Controls continued to receive saline. (B) Cadmium and nickel both caused a significant increase in the size of the AHP amplitude (Controls: n = 8; Cadmium: n = 8; Nickel: n = 8; One‐way ANOVA with Holm–Sidak pairwise multiple comparisons, df = 2, F = 19.6, P < 0.001). (C) Cadmium and nickel significantly increased the recovery period from the AHP compared to nickel (One‐way ANOVA on Ranks with Student–Neuman–Keuls method pairwise multiple comparisons, df = 2, H = 18.7, P < 0.001). (B) Data are plotted as mean and SE. (C) Data are plotted as median and upper and lower quartile. Letters indicate significance (P < 0.05).