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. 2016 May 24;4(10):e12814. doi: 10.14814/phy2.12814

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© 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Calcium removal increases CV in the DCMD at high temperature. (A) Representative relative CV profiles at 35°C show opposite effects of calcium free (calcium‐free saline with 1 mmol/L EGTA) and high calcium (saline with 8 mmol/L calcium) at 20 and 50 min of exposure. (B) Calcium‐free saline (n = 6) produced a significant increase in CV across all frequencies compared to controls (n = 7) at 20 and 50 min (P < 0.05). High calcium (n = 5) produced an initial reduction in CV at 20 minutes across most frequencies but after 50 min, controls had slowed to equal high calcium in all but the >400 Hz band (Two RM ANOVA with Holm–Sidak pairwise multiple comparisons, df = 2, F > 18 for all frequencies, P < 0.001 for all frequencies). Data are plotted at mean and SE. Letters indicate significant differences (P < 0.05).