. Author manuscript; available in PMC: 2016 May 31.

Published in final edited form as: J Am Chem Soc. 2015 Dec 10;137(50):15742–15752. doi: 10.1021/jacs.5b07910

Table 2.

Relative Stability of T2 Pseudoknot and Its Variants Revealed by Their Barrier Energy Differences

	ΔG (kcal·mol⁻¹)
5′-PK vs 5′-PK-mut	−0.86^a
3′-PK vs 3′-PK-mut	−0.84^a
5′-PK vs 2H	−1.3^b
3′-PK vs 2H	−2.0^b

The unfolding barrier difference with respect to the unfolding of wild-type (WT) pseudoknot. ΔG = −RT ln(τ^WT/τ^mut). τ^WT and τ^mut were Level-1 block duration of WT and mutant pseudoknots.

The unfolding barrier difference with respect to the unfolding of 2H. ΔG = -RT ln(τ/τ^2H), where τ^2H is the block duration of 2H and τ is the block duration of 5′- and 3′-PK.