Table 1. Protection of C57BL/6 mice immunized in a 3-shot regimen with the indicated strains against challenge with wildtype P. berghei ANKA sporozoites or blood stages).

Exp.	Attenuated parasite line (P. berghei ANKA)	1st shot	2nd and 3rd shots* (day of shots)	Challenge dose# (day of challenge)		Protected/challenged animals (pre-patency)+
				Challenge 1	Challenge 2
1	LISP2(–)	10.000	10.000 (d14, d28)	1.000 (d42)	10.000 (d116)	C1: 3/4 (d 6), C2: 1/1
					5.000 bs (d116)	C2: 0/2 (d 4.5)
				1.000		0/4 (d 3)
2	LISP2 (–)	50.000	20.000 (d14, d28)	10.000 (d42)	10.000 (d102)	C1 : 4/4, C2 : 2/2
					10.000 bs(d102)	C2 : 0/2
	uis3(–)	50.000	20.000 (d14, d28)	10.000 (d42)	10.000 (d102)	C1: 6/6**, C2: 6/6
	LISP2(–)/uis3(–)	50.000	20.000 (d14, d28)	10.000 (d42)	10.000 (d102)	C1: 8/8, C2: 8/8
				10.000		0/8 (d 3.1)

Groups of C57BL/6 age-matched mice were used for different immunizations. Blood stages were included to probe stage specific protection.

^*Data are presented as numbers of sporozoites for first, second and third shot; days of shots are indicated in parentheses.

^#mice are challenged with PbANKA wild type sporozoites or blood stages (bs); days of challenge after prime are indicated in parentheses.

⁺C1: challenge 1, C2: challenge 2; day of blood stage emergence (pre-patency) is indicated in parentheses.

^**Two mice became positive after priming; thus challenge was only performed with 6 mice.