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. 2016 May 31;6:26715. doi: 10.1038/srep26715

Figure 1. Concentration-dependent inhibition of OAT1- and OAT3-mediated fluorescein uptake by anionic uremic toxins.

Figure 1

(a–h) A concentration range of eight uremic toxins were exposed to matured ciPTEC-OAT1 (•) and -OAT3 (o) in the presence of 1 μM fluorescein, a known OAT model substrate. The intracellular fluorescent signal was detected by measuring samples at excitation wavelength 485 nm and emission wavelength 535 nm. Blank data were subtracted and data were normalized to control (absence of uremic toxin). Nonlinear regression analysis was performed using Graphpad Prism 5.02. Data are shown as mean ± S.E.M. of three independent experiments performed, at least, in duplicate.