Table 1. Uremic toxins inhibit OAT1- and OAT3-mediated fluorescein uptake.
| Uremic toxin | Cm (μM) in ESRD patients1 | ciPTEC-OAT1 (IC50 - μM) | R square | ciPTEC-OAT3 (IC50 - μM) | R square |
|---|---|---|---|---|---|
| Indoxyl sulfate | 110 | 25 ± 4 | 0.92 | 83 ± 41 | 0.42 |
| l-Kynurenine | 6 | 65 ± 8 | 0.92 | 219 ± 66 | 0.54 |
| Kynurenic acid | 1 | 6 ± 1 | 0.95 | 6 ± 1 | 0.83 |
| Indole-3-acetic acid | 4 | 19 ± 2 | 0.93 | 148 ± 60 | 0.49 |
| Hippuric acid | 398 | 5 ± 1 | 0.95 | 22 ± 9 | 0.78 |
| Indoxyl-β-glucuronide | 9 | 492 ± 68 | 0.67 | 527 ± 218 | 0.55 |
| p-Cresylglucuronide | 44 | 2650 ± 922 | 0.28 | 588 ± 81 | 0.36 |
| p-Cresylsulfate | 675 | 79 ± 14 | 0.84 | 112 ± 19 | 0.80 |
The eight tested anionic uremic toxins inhibit OAT1- and OAT3-mediated fluorescein uptake in a concentration-dependent manner. Mean plasma levels of uremic toxins in ESRD patients were extracted from Duranton et al.6. R square: goodness of fit values extracted from non-linear regression analysis.