Figure 5.

PI3K/Akt/HIF-1α signals mediate insufficient RFA-induced in vivo growth of NSCLC cells (A) Average tumor volume is shown for the A549 tumors. Parental A549 tumors with PBS (n = 6), A549-H tumors with PBS (n = 6), and A549-H tumors with LY (LY294002, n = 6) or YC (YC-1, n = 6). (B) Mice were killed 34 days after implantation and the tumor tissues were removed and measured. (C) The protein levels of p-Akt, Akt, and HIF-1α were determined by western blot analysis in the tumor lysates. (D) The tumor tissue sections of each group were subjected to immunohistochemistry detection of p-Akt and HIF-1α.