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. 2016 May 31;6:26906. doi: 10.1038/srep26906

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Copyright © 2016, Macmillan Publishers Limited

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NAT activity in the S9 fractions (9.60 μg protein) from untreated rats was determined in vitro as described in methods (A). The inhibitory potency of apocynin (0.49 mM) was tested at different assay conditions: in assays (B–D) apocynin was preincubated with S9 fractions for 0, 5′ or 15′ respectively; in assays E and F, S9 fractions were preincubated with AcCoA for 5′ or 15′ respectively. Appropriate enzymatic control assays were performed for each condition. Assays (B–F) were all statistically different from assay A (p < 0.05); ** denotes statistically significant difference (p < 0.001) between assays E and F.