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. Author manuscript; available in PMC: 2016 May 31.
Published in final edited form as: Immunobiology. 2014 Aug 10;219(12):924–931. doi: 10.1016/j.imbio.2014.08.001

Figure 1. Hsp90-Ova can interact with SREC-I at the cell surface and in intracellular compartments.

Figure 1

A and B, Hsp90-PL19 can interact with SREC-I in Raw264.7 cells. Raw 264.7 cells were transfected with a construct expressing FLAG-SREC-I for 18 hours. Cells were then incubated with Alexa 555-Hsp90-PL19 peptide (10ug/ml) complexes on ice for 20 minutes followed by incubation at 37°C for 10 minutes. The cells were then permeabilized for 5 minutes and then fixed, stained for FLAG with anti-FLAG ab (green). C, KG1 cells (DC cell line) were transfected with FLAG-SREC-I and then incubated with Alexa 555-Hsp90.Ova for 5–10 mins. Cells were then fixed and either permeabilized (D) or not (C) with 4% PFA. Scale bar 5μm.