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. Author manuscript; available in PMC: 2016 May 31.
Published in final edited form as: Immunobiology. 2014 Aug 10;219(12):924–931. doi: 10.1016/j.imbio.2014.08.001

Figure 4. Presentation of Hsp90-chaperoned antigens after binding SREC-I requires actin, Rho-GTPase activity.

Figure 4

Immature BMDCs were treated with Toxin B (2 ng/ml) and incubated at 37°C. Cells were then pulsed with OVA polypeptides, Hsp90-PC complexes, and anti–SREC-I-Ab-OVA for 2h at 37°C, fixed, washed, then cultured with KZO T cell hybridoma cells overnight. IFN-γ secreted by KZO cells was measured by sandwich ELISA using anti–IFN-γ Ab. Data shown are the means + SD of triplicate assays. The data shown are representative of at least three independent experiments. Scale bar 5μm.