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. 2016 May 30;213(6):1047–1059. doi: 10.1084/jem.20151000

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© 2016 Zhu et al.

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Figure 4. — Microglia-deficient IL34^−/− mice displayed similar microglial activation, but augmented astrogliosis. (A, left) Iba1 immunohistochemistry of brain tissues from RML6-infected IL34^−/− and IL34^+/+ (WT) mice at 105 dpi. (right) Quantification of the Iba1 immunostaining. n = 4; n.s, P > 0.05. Bars, 20 µm. (B, left) Iba1 immunohistochemistry of brain tissues from RML6-infected IL34^−/− and IL34^+/+ (WT) mice at 150 dpi. (right) Quantification of the Iba1 immunostaining. n = 3; n.s, P > 0.05. Bars, 20 µm. (C, left) Iba1 Western blot of one hemisphere from RML6-infected IL34^−/− and IL34^+/+ (WT) mice at 105 dpi. (right) Densitometric quantification of the Iba1 Western blot. n = 4; n.s, P > 0.05. (D, left) Iba1 Western blot of one hemisphere from RML6-infected IL34^−/− and IL34^+/+ (WT) mice at 150 dpi. (right) Densitometric quantification of the Iba1 Western blot. n = 5; n.s, P > 0.05. (E–J) qRT-PCR of cytokines from one hemisphere of RML6-infected IL34^−/− and IL34^+/+ (WT) mice at 105 dpi (E–G) and 150 dpi (H–J). (E and H) TNF mRNA, (F and I) IL-1β mRNA, and (G and J) IL-6 mRNA. Relative expression normalized to GAPDH expression and represented as fold change compared with WT mice ± SEM. n = 4 (105 dpi) or 5 (150 dpi) for IL34^−/−; n = 4 (105 dpi) or 6 (150 dpi) for IL34^+/+, n.s, P > 0.05. (K, left) GFAP immunohistochemistry of brain tissues from RML6-infected IL34^−/− and IL34^+/+ (WT) mice at 105 dpi. (right) Quantification of the GFAP immunostaining, n = 4, *, P < 0.05. Bars, 20 µm. (L, left) GFAP immunohistochemistry of brain tissues from RML6-infected IL34^−/− and IL34^+/+ (WT) mice at 150 dpi. (right) Quantification of the GFAP immunostaining. n = 3; *, P < 0.05. Bars, 20 µm. (M, left) GFAP Western blot of one hemisphere from RML6-infected IL34^−/− and IL34^+/+ (WT) mice at 105 dpi. (right) Densitometric quantification of the GFAP Western blot. n = 4 for IL34^−/−; n = 4 for IL34^+/+, *, P < 0.05. (N, left) GFAP Western blot of one hemisphere from RML6-infected IL34^−/− and IL34^+/+ (WT) mice at 150 dpi. (right) Densitometric quantification of the GFAP Western blot. n = 5, **, P < 0.01. Error bars represent SEM. Statistical significance in A–N was determined using unpaired Student’s t test. Histology and Western blot results represent at least three independent experiments.