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. 2016 May 30;213(6):1047–1059. doi: 10.1084/jem.20151000

Figure 5.

Figure 5.

Similar PrPSc deposition, microglial activation, and astrogliosis in terminally sick IL34−/− and IL34+/+ mice. (A) H&E and SAF84 immunohistochemistry of the hippocampi of terminally sick RML6-infected IL34−/− and IL34+/+ (WT) mice. Bars, 50 µm. (B, top) PrPSc Western blot of hemispheric brain homogenates prepared from terminally sick RML6-infected IL34−/− and IL34+/+ (WT) mice. (bottom) Densitometric quantification of the PrPSc Western blot. n = 5. (C) Iba1 immunohistochemistry of hippocampi and cortex from terminally sick RML6-infected IL34−/− and IL34+/+ (WT) mice. Bars, 20 µm. (D, top) Iba1 Western blot of hemispheric brain homogenates prepared from terminally sick RML6-infected IL34−/− and IL34+/+ (WT) mice. (bottom) Densitometric quantification of the Iba1 Western blot. n = 5. (E) GFAP immunohistochemistry of hippocampi and cortex from terminally sick RML6-infected IL34−/− and IL34+/+ (WT) mice. Bars, 20 µm. (F, top) GFAP Western blot of hemispheric brain homogenates prepared from terminally sick RML6-infected IL34−/− and IL34+/+ (WT) mice. (bottom) Densitometric quantification of the GFAP Western blot. n = 5. Error bars represent SEM. Statistical significance in B, D, and F was determined using unpaired Student’s t test. Histology and Western blot results represent at least five independent experiments.