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. 2016 May 30;213(6):1011–1028. doi: 10.1084/jem.20151183

Figure 4.

Figure 4.

Identification of Hebo. (A) Schematic representation of the annotated ERCC6L2 gene in human (top) and rat (bottom) as provided by the Ensembl database. Exons 1–14 correspond to the human SF of ERCC6L2. A splice donor site within exon 14 would lead to alternative splicing from transcript 001 to transcript 007, incorporating new exon 15 and part of exon 16. Annotation is interrupted in exon 16 in transcript 007. Transcript 006 would incorporate the end of exon 16 onward to the end of the putative cDNA at exon 19 when compared with the structure of the rat gene. A gap of 347 bp between the two parts of exon 16 present in transcripts 007 and 006, respectively, was filled in through PCR amplification of cDNA. The existence of the ERCC6L2 long form (Hebo) was validated through full-length PCR amplification of cDNA. (B) Real-time quantitative RT-PCR of ERCC6L2-SF and Hebo-specific transcripts in a panel of tissues. This experiment was performed two times. Data are mean ± SEM. (C) Cellular localization of GFP-Hebo and GFP–ERCC6L2-SF in 293T-transfected cells. This experiment was performed three times. Bar, 10 µm.