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. 2016 May 30;213(6):897–911. doi: 10.1084/jem.20151715

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© 2016 Scott et al.

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Figure 2. — Zeb2 expression differentially affects subsets of tissue-resident cDC2s. (A, C, and E) Representative FACS plots showing identification of XCR1⁺SIRPα⁻ cDC1s and XCR1⁻SIRPα⁺ cDC2s in the liver (A), lung (C), and SI LP (E) of Zeb2^+/+, Zeb2^+/−, Zeb2^−/−, Zeb2^+/Tg, and Zeb2^Tg/Tg mice. Cells were pregated as single live CD45⁺ lineage⁻CD64⁻F4/80⁻MHCII⁺CD11c⁺CD26⁺. The numbers represent the proportion of cDC1s and cDC2s as a percentage of total cDCs. (B, D, and F) Proportion of liver (B), lung (D), and SI LP (F) cDC1s and cDC2s as a percentage of total cDCs in Zeb2^+/+, Zeb2^+/−, Zeb2^−/−, Zeb2^+/Tg, and Zeb2^Tg/Tg mice. Data are pooled from two to three experiments, with at least n = 7 per group. (G) Representative FACS plots showing identification of CD103⁺SIRPα⁻ cDC1s, CD103⁺SIRPα⁺ cDC2s, and CD103⁻SIRPα⁺ cDC2s in the SI LP of Zeb2^+/+, Zeb2^+/−, Zeb2^−/−, Zeb2^+/Tg, and Zeb2^Tg/Tg mice. Cells were pregated as single live CD45⁺ lineage⁻CD64⁻F4/80⁻MHCII⁺CD11c⁺CD26⁺. The numbers represent the proportion of each cDC subset as a percentage of total cDCs. (H) Proportion of SI LP CD103⁺ cDC1s, CD103⁺ cDC2s, and CD103⁻ cDC2s as a percentage of total cDCs in Zeb2^+/+, Zeb2^+/−, Zeb2^−/−, Zeb2^+/Tg, and Zeb2^Tg/Tg mice. Data are pooled from two to three experiments, with at least n = 7 per group. (I) Proportion of MLN-resident XCR1⁺SIRPα⁻ cDC1s, XCR1⁻SIRPα⁺ cDC2s, and XCR1⁺SIRPα⁺ DP cDCs as a percentage of total resident cDCs in Zeb2^+/+, Zeb2^−/−, and Zeb2^Tg/Tg mice. Data are representative of two experiments, with n = 5 per group. (J) Proportion of MLN migratory CD103⁺ cDC1s, CD103⁺ cDC2s, and CD103⁻ cDC2s as a percentage of total migratory cDCs in Zeb2^+/+, Zeb2^−/−, and Zeb2^Tg/Tg mice. Data are representative of two experiments, with n = 5 per group. *, P < 0.05; **, P < 0.01; ***, P < 0.001. One-way ANOVA with Bonferroni posttest was used.