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. 2016 May 31;13:126. doi: 10.1186/s12974-016-0594-7

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Depletion of calcium stores from endoplasmic reticulum induces STIM1 puncta formation in spinal astrocytes. a Live-cell confocal time-lapse images of STIM1-transfected astrocytes treated with 1 μM thapsigargin (TG) or 30 μM cyclopiazonic acid (CPA) at 0, 4, and 8 min. b Average number of puncta per 100 μm² induced by respective treatments. Puncta were quantified as spots of high fluorescence intensity ranging from 0.4 to 2.0 μm in diameter size. c Confocal images of fixed spinal astrocytes containing endogenous STIM1 puncta after 8 min in the presence and absence of TG (1 μM)