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. Author manuscript; available in PMC: 2016 May 31.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 May 30;33(8):2004–2012. doi: 10.1161/ATVBAHA.112.301166

Figure 4.

Figure 4

In vitro proliferation, motility, and migration of muscle-derived stem cells (MDSCs) were seeded onto collagen-coated plates and put on a live cell imager. Bright field pictures were analyzed every 12 hours to determine cellular proliferation. A, Proliferation under normal conditions in proliferation media (n=12; *P<0.05 to nonstimulated [NS] sFlt1-MDSC; &P<0.05 to mechanical stimulation [MS] sFlt1-MDSC). B, Proliferation under oxidative stress conditions of 250 μmol/L H2O2 in proliferation media. C, Motility under normal and oxidative stress conditions. No significant differences between groups under normal conditions or under oxidative stress conditions (n=12; *P<0.05 between MS sFlt1-MDSCs grown under normal and oxidative stress conditions and between MS short hairpin RNA (shRNA)_vascular endothelial growth factor (VEGF) MDSC groups under normal and oxidative stress conditions). D, NS lacZ-MDSCs closed a scratch wound more rapidly than all other groups (n=6; *P<0.05). There was a significant difference between shRNA_VEGF MS and NS wound closure (&P<0.05). lacZ-MDSC indicates MDSCs were transduced with retroviral vectors encoding the LacZ reporter gene; and sFlt1- MDSC, MDSCs were transduced with retroviral vectors encoding the soluble VEGF receptor Flt1.