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. 2016 May 31;6:26874. doi: 10.1038/srep26874

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(a) Immunostaining for Sox9 (red), YFP (green) on control (left) and RBP-Jκ (right) E15.5 pancreatic rudiments. The lower line presents enlargements of selected areas. (b) Immunostaining for Sox9 (red), YFP (green) on Ngn3-Cre; YFP (left) and Ngn3-Cre; RBP-Jκ^f/f; YFP (right) pancreatic anlages at E18.5. The lower line presents enlargements of selected areas. (c) Immunostaining for Sox9 (red), YFP (green) on disorganized areas of adult Ngn3-Cre; RBP-Jκ^f/f; YFP mice (left). The right panel presents enlargements of selected areas. Arrows illustrate YFP+ cells expressing high levels of Sox9. All nuclei were counterstained in blue with DAPI. Scale bars = 50 μm.