Table 2.

Primer oligonucleotides sequence for detection Brucella spp. and antibiotics resistance gene. (Sorted by amplification target length)

Target	Primers	Length
Brucella spp.	5′GCGTTGGAGGCGACATTGACA3′ 5′AGGCTGGCGAGGCGGATG3′	286
	Tetracycline Resistance Genes
tet(W)	5′ACGGCTGCTGGACGCTCTT3′ 5′ATGGTGTGGCTGGCTGCTTTG3′	219
tet(L)	5′CCACCTGCGAGTACAAACTGGG3′ 5′ATCGCTGGACCGACTCCTTCTC3′	335
tet(P)	5′GCTCCCTCTGCTGCAAACAACT3′ 5′TCCCAACGGAAGCGGTGATACA3′	340
tet(S)	5′CCCTGTCAGTACGCCAGCAGAT3′ 5′TTGGACGACGTGGTTGGAAAGC3′	350
tet(B)	5′ATCGGGTCCCTGGTAGCAATGG3′ 5′AGTCCTCCGCAAAGGGTTCCAA3′	371
tet(A)	5′TCGTCGCCGCCCTGATGG3′ 5′GCCGCATAGATCGCCGTGAAG3′	451
tet(C)	5′CGCAGTCAGGCACCGTGTATG3′ 5′CGCCGCCGCAAGGAATGG3′	519
tet(O)	5′TTCTGGGCTTCTGTCGGGTTGT3′ 5′CTATTCGGGCGGCGGGGTT3′	557
tet(M)	5′CGAGGTCCGTCTGAACTTTGCG3′ 5′GCGGCACTTCGATGTGAATGGT3′	583
	Chloramphenicol Resistance Gene
catB	5′AGCCTCTCAGCGAACAGGTCAA3′ 5′AGTCCCACCACGCCATTTCCA3′	507
	Aminoglycoside Resistance Genes
Aac	5′TGGAGCACTGGCGCGGATT3′ 5′GAAAACCACGGGCGAACTGTCA3′	200
aac(6′)-Ib	5′AGAAGAAGCACGCCCGACACT3′ 5′GTTCCCAAGCCTTTGCCCAGTT3′	237
aac(3)-Ia	5′GAGTTCGGAGACGTAGCCACCT3′ 5′ATAGAGAGCCACTGCGGGATCG3′	324
aac(6′)-Ib-cr4	5′CAACAGCACCGATTCCGTCACA3′ 5′GTTCCCAAGCCTTTGCCCAGTT3′	339
ant(3″)-Ia	5′TCAGAGGTAGTTGGCGTCATCG3′ 5′GCGGCGAGTTCCATAGCGTTA3′	490