Table 1. Overview of western blot quantification performed on the Camk2b mutants in percentage of controls.
| T287A |
CAG-CreER |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Hippocampus | Cortex | Hippocampus | Cerebellum | |||||||
| Antibody | WT (4) | T287A (4) | Cre− (3) | Cre+(5) | Cre− (3) | Cre+(5) | Cre− (3) | Cre+(5) | ||
| CAMK2B | 100 ± 27 | 84 ± 22 | 100 ± 16 | 0 ± 0 | 100 ± 15 | 0.2 ± 0.1 | 100 ± 13 | 0 ± 0 | ||
| CAMK2A | 100 ± 24 | 67 ± 11 | 100 ± 7 | 126 ± 25 | 100 ± 10 | 89 ± 3 | 100 ± 13 | 57 ± 20 | ||
| Ph-T287 | 100 ± 16 | 0.1 ± 0.1 | ||||||||
| Ph-T286 | 100 ± 11 | 116 ± 18 | ||||||||
| Δex2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Cortex | Hippocampus | Cerebellum | ||||||||
| Antibody | WT (2) | Het (2) | KO (2) | WT (8) | Het (8) | KO (2) | WT (4) | Het (4) | KO (4) | |
| CAMK2B | 100 ± 16 | 23 ± 8 | 2 ± 1 | 100 ± 5 | 13 ± 2 | 10 ± 4 | 100 ± 5 | 17 ± 5 | 6 ± 2 | |
| CAMK2A | 100 ± 25 | 109 ± 35 | 118 ± 45 | 100 ± 7 | 96 ± 13 | 128 ± 34 | 100 ± 16 | 64 ± 21 | 77 ± 28 | |
| L7-Cre | GABAa6-Cre | RGS9-Cre | EMX-Cre | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Cerebellum | Cerebellum | Striatum | Cortex | Hippocampus | ||||||
| Antibody | Cre− (2) | Cre + (2) | Cre− (5) | Cre + (5) | Cre− (4) | Cre + (4) | Cre− (6) | Cre + (6) | Cre− (6) | Cre + (6) |
| CAMK2B | 100 ± 11 | 19 ± 3 | 100 ± 17 | 9 ± 1 | 100 ± 12 | 19 ± 4 | 100 ± 12 | 2 ± 1 | 100 ± 8 | 0 ± 0 |
| CAMK2A | 100 ± 15 | 30 ± 1 | 100 ± 9 | 115 ± 7 | 100 ± 17 | 104 ± 15 | 100 ± 23 | 92 ± 7 | 100 ± 8 | 87 ± 6 |
| L7-Cre | ||||
|---|---|---|---|---|
| Cerebellum | ||||
| Antibody | WT (4) | Cre+(2) | WT (4) | Cre− (2) |
| CAMK2B | 100 ± 23 | 27 ± 5 | 100 ± 23 | 139 ± 15 |
| CAMK2A | 100 ± 7 | 18 ± 1 | 100 ± 7 | 61 ± 15 |
WT = wildtype; Het = heterozygous knockout; KO = knockout. Cre– = Cre-negative Camk2b mutant; Cre + = Cre-positive Camk2b mutant. Number of samples is depicted in brackets.