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. 2016 May 31;6:26993. doi: 10.1038/srep26993

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(a) Recombinant protein expression and purification. The arrowhead indicates the specific bands for the purified PvRBP1a-34 (34.4 kDa) and PvRBP1b-32 (32.0 kDa) recombinant proteins. T, total translation mix; S, supernatant; P, pellet; Ft, flow-through; E, elution. (b) The purified recombinant PvRBP1a-34 and PvRBP1b-32 proteins were probed with the anti-penta-His antibody (His), immune mouse sera (M), immune rabbit sera (R) and preimmune rabbit sera (N) under reducing conditions as well as pooled patient sera from 10 vivax malaria patients (P) and pooled sera from 10 healthy people (H). (c) Recognition of the native PvRBP1a and PvRBP1b antigens in the P. vivax schizont parasite lysate with rabbit antisera raised against the recombinant PvRBP1a-34 and PvRBP1b-32 proteins under reducing conditions. (1) Native PvRBP1a with rabbit immune sera; (3) native PvRBP1b with rabbit immune sera; (2) and (4) represent normal RBC extracts with PvRBP1a and PvRBP1b rabbit immune sera, respectively. The arrowhead represents the target bands and their processed fragments.