Table 3.
Validation parameters for AM-2201 and metabolites: method recovery, and matrix effect. Low and high quality control working solutions contained all 14 standards at 3 (low) and 400 (high) μg/L for AM-2201 N-(4-hydroxypentyl), JWH-018 N-propanoic acid, and JWH-073 N-(2-hydroxybutyl) and 1.5 and 400 μg/L for other analytes.
| Compound | Recovery (%) |
Matrix effect (post peak areas CV) (%) |
||
|---|---|---|---|---|
| Low (n = 10) |
High (n = 10) |
Low (n = 10) |
High (n = 10) |
|
| AM-2201 | 65.7 | 61.3 | −57.6 (64.0) | −47.7 (65.0) |
| AM-2201 N-(4-hydroxypentyl) | 75.7 | 84.4 | −37.3 (55.7) | −35.8 (52.7) |
| AM-2201 6′-hydroxyindole | 67.7 | 70.4 | −46.0 (61.0) | −42.1 (59.2) |
| AM-2201 7′-hydroxyindole | 65.2 | 58.4 | −62.1 (85.2) | −55.5 (83.8) |
| JWH-018 N-(2-hydroxypentyl) | 69.2 | 71.1 | −44.2 (62.8) | −38.4 (60.4) |
| JWH-018 N-(3-hydroxypentyl) | 71.4 | 76.7 | −45.2 (59.3) | −40.9 (55.9) |
| JWH-018 N-(4-hydroxypentyl) | 75.8 | 81.5 | −35.3 (48.7) | −21.5 (47.0) |
| JWH-018 N-(5-hydroxypentyl) | 74.0 | 79.4 | −33.3 (54.1) | −23.8 (53.5) |
| JWH-018 N-pentanoic acid | 73.3 | 79.0 | −22.9 (47.1) | −16.4 (45.6) |
| JWH-018 N-propanoic acid | 73.4 | 78.5 | −32.5 (34.5) | −30.4 (34.3) |
| JWH-073 N-(2-hydroxybutyl) | 69.0 | 80.5 | −37.6 (52.9) | −32.1 (49.1) |
| JWH-073 N-(3-hydroxybutyl) | 78.5 | 81.4 | −46.7 (49.1) | −38.7 (46.5) |
| JWH-073 N-(4-hydroxybutyl) | 77.5 | 84.4 | −32.5 (44.9) | −28.6 (41.9) |
| JWH-073 N-butanoic acid | 74.4 | 78.6 | −26.6 (37.9) | −15.6 (34.7) |