Figure 3.

Effect of ghrelin administration (2.0mg/kg, s.c.) during stronger stress (air-puff in all corners) in a social environment (Intellicage system). Ghrelin increased visit number (a) and visit duration (b) during 3 hours of air-puff punishment in all corners in the Intellicage system, indicating a stress-attenuating effect of ghrelin (n=10). Because of the stronger stress, the mice displayed rather few visits, and we consequently had to analyze data over a longer period (3 hours) in this experiment compared with the mild stress experiment (Figure 2 a-c). Asterisks indicate statistical significance in unpaired Student’s t test (*P≤.05).