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. 2015 Nov 17;19(5):pyv123. doi: 10.1093/ijnp/pyv123

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© The Author 2015. Published by Oxford University Press on behalf of CINP.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Verification of recombinant adeno-associated viral vector (rAAV)-mediated overexpression of Ghrelin receptor (Ghr-R) in the basolateral division of the amygdala (BL-d). (a-c) In situ hybridization shows Ghr-R mRNA expression in the lateral (La) and basolateral (BLA) nuclei of Ghr-R-overexpressing mice. (d) c-fos mRNA expression is significantly increased in the BL-d in Ghr-R-overexpressing mice compared with controls (quantified from in situ hybridization, n=8–12). (e) Timeline of Ghr-R mRNA expression after rAAV surgery showing a peak after 2 weeks, which is stable until 4 weeks after surgery (n=3). Scale bars=0.5mm. Asterisks indicate statistical significance in unpaired Student’s t test (d) or compared with 1 week after rAAV surgery in Dunnett’s posthoc test following significant 1-way ANOVA (e) (*P<.05, ***P<.001).