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. 2015 Nov 17;19(5):pyv123. doi: 10.1093/ijnp/pyv123

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© The Author 2015. Published by Oxford University Press on behalf of CINP.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Effect of intra-basolateral division of the amygdala (BL-d) recombinant adeno-associated viral vector (rAAV)-mediated Ghrelin receptor (Ghr-R) overexpression on expression of corticotropin-releasing factor (CRF) and 5-HT1a, 5-HT2c, 5-HT5a, and 5-HT7 receptor mRNA in the BL-d in mice subjected to restraint stress. Ghr-R overexpression significantly decreased 5-HT1a expression (a) but had no significant effect on the expression of 5-HT2c, 5-HT5a, 5-HT7, or CRF (b-e) (n=11–12). Levels of mRNA were calculated relative to the mean CT value of the reference genes TATA-box binding protein (TBP) and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta (YWHAZ); empty vector control levels were set to 100. Asterisk indicates statistical significance in unpaired Student’s t test (*P<.05).