Table 2.
Effect of Ghrelin Injections on the HPA Axis and Ghrelin Plasma Levels
| C57BL/6 Females Saline | C57BL/6 Females Ghrelin (2mg/kg s.c.) | NMRI Males Saline | NMRI Males Ghrelin (3mg/kg i.p.) | |
|---|---|---|---|---|
| Ghrelin (active) (pg/mL) | 97.6±17.9 | 708 ±81.5#### | 52.1±10.2 | 1769±293*** |
| Ghrelin (total) (pg/mL) | 338±31.4 | 3646±153#### | 194±34.8 | 11801±1708**** |
| ACTH (pg/mL) | 455±14.0 | 440±10.9 | 465±43.9 | 494±23.7 |
| Corticosterone (ng/mL) | 256±7.74 | 281±9.2 | 95.7±9.8 | 160±8.5 *** |
Abbreviations: ACTH: adrenocorticotropic hormone.
Acute effect of ghrelin in C57Bl/6 female mice (2mg/kg s.c.) and in male NMRI mice (3mg/kg i.p.) on total and active ghrelin, ACTH and corticosterone levels measured 30min after injection (C57Bl/6 females) or 15 minutes after injection (NMRI males). n=8/group. Data tested with unpaired two-tailed t-test. (#### P<.0001 C57Bl/6 females saline vs ghrelin, ***P<.001, ****P<.0001 NMRI males saline versus ghrelin).