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. 2016 May 31;6:27071. doi: 10.1038/srep27071

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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A549 cells were transfected with tandem fluorescent mRFP-GFP-tagged LC3 plasimd (ptfLC3) for 24 h. Then the transfected cells were treated with 1 μM of Stel B for 0, 3, 6, 17, 24 h, followed by confocal fluorescence microscopy. Arrows indicate autophagosomes. Bar = 10 μm. Data represent three independent experiments.