Table 1.
Purification of Enterocins DD 28 and DD93.
| Strains | Purification stages | Supernatant volume (ml) | Activity AU/ml | Protein (mg/ml) | Total protein (mg) | Total activity | Specific activity (AU/mg) | Purification factor |
|---|---|---|---|---|---|---|---|---|
| Enterococcus faecalis 28 | Supernatant | 1 000 | 800 | 9.28 | 9 280 | 800 000 | 86.20 | 1 |
| Sephadex CM 25 | 50 | 3 200 | 0.82 | 41 | 160 000 | 3 902.44 | 45.27 | |
| C18 RP-HPLC | 1 | 6 400 | 0.095 | 0.095 | 6 400 | 67 368.42 | 781.53 | |
| E. faecalis 93 | Supernatant | 1 000 | 800 | 8.32 | 8 320 | 800 000 | 96.15 | 1 |
| Sephadex CM 25 | 50 | 1 600 | 0.69 | 34.5 | 80 000 | 2 318.84 | 24.11 | |
| C18 RP-HPLC | 1 | 6 400 | 0.083 | 0.083 | 6 400 | 77 108.43 | 801.96 | |
Protein concentration was determined by BiCinchoninic acid Assay (BCA) kit and the manufacturer recommendations (Sigma–Aldrich, USA). Antibacterial activity was measured against L. innocua CIP80.11 as indictor strain.