TABLE 1.
Strains used in this study
| Strain | Descriptiona | Source or reference(s) |
|---|---|---|
| E. coli | ||
| DH5αmcr | Strain used for general cloning | Life Technologies (Grand Island, NY) |
| EC100D pir | Strain used to clone HimarEm1 and adjacent DNA from C. algicola Himar mutants | Epicentre (Madison, WI) |
| S17-1 λ pir | Strain used for conjugation | 51 |
| HB101 | Strain used with pRK2013 for triparental conjugation | 22, 52 |
| F. johnsoniae | ||
| CJ1827 | rpsL2 Smr; wild-type F. johnsoniae strain used in construction of deletion mutants | 26 |
| CJ2157 | rpsL2 ΔgldL Smr | 18 |
| C. algicola | ||
| CA2 | Wild-type DSM 14237T | DSMZ |
| CA4 | HimarEm1 insertion mutation in sprE (Celal_1548) of strain CA2; Emr | This study |
| CA19 | HimarEm1 insertion mutation in lolA (Celal_3622) of strain CA2; Emr | This study |
| CA24 | HimarEm1 insertion mutation in sprT (Celal_2247) of strain CA2; Emr | This study |
| CA32 | HimarEm1 insertion mutation in gldM (Celal_0882) of strain CA2; Emr | This study |
| CA33 | HimarEm1 insertion mutation in gldJ (Celal_0751) of strain CA2; Emr | This study |
| CA51 | HimarEm1 insertion mutation in sprA (Celal_2075) of strain CA2; Emr | This study |
| CA65 | HimarEm1 insertion mutation in gldK (Celal_0884) of strain CA2; Emr | This study |
| CA69 | HimarEm1 insertion mutation in gldN (Celal_0881) of strain CA2; Emr | This study |
| CA72 | HimarEm1 insertion mutation in sprF (Celal_1477) of strain CA2; Emr | This study |
| CA89 | HimarEm1 insertion mutation in sprC (Celal_1480) of strain CA2; Emr | This study |
| CA92 | HimarEm1 insertion mutation in gldL (Celal_0883) of strain CA2; Emr | This study |
| CA94 | HimarEm1 insertion mutation in gldB (Celal_2202) of strain CA2; Emr | This study |
| CA97 | HimarEm1 insertion mutation in gldI (Celal_2755) of strain CA2; Emr | This study |
| CA119 | gldD (Celal_1091) disruption in strain CA2; Emr | This study |
| CA121 | gldH (Celal_1514) disruption in strain CA2; Emr | This study |
| CA124 | sprD (Celal_1479) disruption in strain CA2; Emr | This study |
| CA130 | ΔgldD in strain CA2 | This study |
| CA135 | rpsLA168G in strain CA2; Smr wild-type strain used for construction of deletion mutations | This study |
| CA143 | ΔsprBI (Celal_1478) rpsLA168G Smr | This study |
| CA149 | ΔgldB rpsLA168G Smr | This study |
| CA150 | ΔgldL rpsLA168G Smr | This study |
| CA179 | ΔgldB ΔgldL rpsLA168G Smr | This study |
| CA184 | Δ(sprC-sprD) rpsLA168G Smr | This study |
| CA185 | Δ(sprC-sprD) ΔgldL rpsLA168G Smr | This study |
| CA239 | ΔsprBII (Celal_0324) rpsLA168G Smr | This study |
| CA240 | ΔsprBI ΔsprBII rpsLA168G Smr | This study |
| CA241 | Δ(gldK-gldN) rpsLA168G Smr | This study |
a
Emr, erythromycin resistant; Smr, streptomycin resistant.