FIG 2.

Facilitated dissociation of proteins from isolated nucleoids. Isolated GFP-Fis-labeled nucleoids were exposed to 500 nM WT Fis in 100 mM NaCl buffer (A) and protein-free 100 mM NaCl buffer (B). A large drop in fluorescence intensity was observed only in the case of 500 nM WT Fis. (C) Fluorescence intensity was measured after 20 min of incubation of nucleoids with protein-free buffer and 50 and 500 nM WT Fis, showing no protein dissociation for protein-free buffer and increased reduction of fluorescence with increased protein concentration. Scale bars are 5 μm.