Structure-based sequence alignment of H5Nx HA. The secondary structure (ss) is highlighted in cyan for the β-sheet and in magenta for the α-helix. The locations equivalent to H1 and H3 antigenic sites are labeled with the antigenic site designation (Sa [1], Sb [2], Ca [3], or Cb [4] for H1 antigenic sites and A, B, C, D, or E for H3 antigenic sites). Residues around the RBS are shaded.