TABLE 2.
Glycan microarray for H5Nx HAs
| Glycan no. by category | Structurea | Bindingb |
||
|---|---|---|---|---|
| A/northern pintail/ Washington/ 40964/14 |
A/Sichuan/ 26221/14 |
A/gyrfalcon/ Washington/ 41088-6/14 |
||
| Sialic acid | ||||
| 1 | Neu5Acα | NB | NB | NB |
| 2 | Neu5Acα | NB | NB | NB |
| 3 | Neu5Acβ | NB | NB | NB |
| α2-3 sialosides | ||||
| 4 | Neu5Acα2-3(6-O-Su)Galβ1-4GlcNAcβ | +++ | +++ | +++ |
| 5 | Neu5Acα2-3Galβ1-3[6OSO3]GalNAcα | +++ | +++ | +++ |
| 6 | Neu5Acα2-3Galβ1-4[6OSO3]GlcNAcβ | +++ | +++ | +++ |
| 7 | Neu5Acα2-3Galβ1-4(Fucα1-3)[6OSO3]GlcNAcβ-propyl-NH2 | +++ | +++ | +++ |
| 8 | Neu5Acα2-3Galβ1-3[6OSO3]GlcNAc-propyl-NH2 | +++ | +++ | +++ |
| 9 | Neu5Acα2-3Galβ1-3(Neu5Acα2-3Galβ1-4)GlcNAcβ | +++ | +++ | +++ |
| 10 | Neu5Acα2-3Galβ1-3(Neu5Acα2-3Galβ1-4GlcNAcβ1-6)GalNAcα | +++ | +++ | +++ |
| 11 | Neu5Acα2-3Galβ1-4GlcNAcβ1-2Manα1-3(Neu5Acα2-3Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ | +++ | +++ | +++ |
| 12 | Neu5Acα(2-3)-Galβ(1-4)-GlcNAcβ(1-3)-Galβ(1-4)-GlcNAcβ(1-2)-Manα(1-3)-[Neu5Acα(2-3)-Galβ(1-4)-GlcNAcβ(1-3)-Galβ(1-4)-GlcNAcβ(1-2)-Manα(1-6)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ | +++ | +++ | +++ |
| 13 | Neu5Acα2-3Galβ | +++ | +++ | ++ |
| 14 | Neu5Acα2-3Galβ1-3GalNAcα | +++ | +++ | +++ |
| 15 | Neu5Acα2-3Galβ1-3GlcNAcβ | +++ | +++ | ++ |
| 16 | Neu5Acα2-3Galβ1-3GlcNAcβ | +++ | +++ | +++ |
| 17 | Neu5Acα2-3Galβ1-4Glcβ | +++ | +++ | +++ |
| 18 | Neu5Acα2-3Galβ1-4Glcβ | +++ | +++ | +++ |
| 19 | Neu5Acα2-3Galβ1-4GlcNAcβ | +++ | +++ | +++ |
| 20 | Neu5Acα2-3Galβ1-4GlcNAcβ | +++ | +++ | +++ |
| 21 | Neu5Acα2-3GalNAcβ1-4GlcNAcβ | NB | NB | ++ |
| 22 | Neu5Acα2-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ | +++ | +++ | +++ |
| 23 | Neu5Aca2-3Galβ1-3GlcNAcβ1-3Galβ1-4GlcNAcβ | +++ | +++ | +++ |
| 24 | Neu5Acα2-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ | +++ | +++ | +++ |
| 25 | Neu5Acα2-3Galβ1-4GlcNAcβ1-3Galβ1-3GlcNAcβ | +++ | +++ | +++ |
| 26 | Neu5Acα2-3Galβ1-3GalNAcα | +++ | +++ | +++ |
| 27 | Galβ1-3(Neu5Acα2-3Galβ1-4(Fucα1-3)GlcNAcβ1-6)GalNAcα | NB | + | NB |
| 28 | Neu5Acα2-3Galβ1-3(Fucα1-4)GlcNAcβ | +++ | +++ | +++ |
| 29 | Neu5Acα2-3Galβ1-4(Fucα1-3)GlcNAcβ | +++ | +++ | +++ |
| 30 | Neu5Acα2-3Galβ1-4(Fucα1-3)GlcNAcβ | +++ | +++ | +++ |
| 31 | Neu5Acα2-3Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ | +++ | +++ | +++ |
| 32 | Neu5Acα2-3Galβ1-3[Fucα1-4]GlcNAcβ1-3Galβ1-4[Fucα1-3]GlcNAcβ | +++ | +++ | +++ |
| 33 | Neu5Acα2-3Galβ1-3[Fucα1-3]GlcNAcβ1-3Galβ1-4[Fucα1-3]GlcNAcβ | +++ | +++ | +++ |
| 34 | Neu5Acα2-3Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ1-4(Fucα1-3)GlcNAcβ | +++ | +++ | +++ |
| 35 | Neu5Acα2-3(GalNAcβ1-4)Galβ1-4GlcNAcβ | NB | NB | NB |
| 36 | Neu5Acα2-3(GalNAcβ1-4)Galβ1-4GlcNAcβ | NB | NB | NB |
| 37 | Neu5Acα2-3(GalNAcβ1-4)Galβ1-4Glcβ | NB | NB | NB |
| 38 | Galβ1-3GalNAcβ1-4(Neu5Acα2-3)Galβ1-4Glcβ | NB | NB | NB |
| 39 | Fucα1-2Galβ1-3GalNAcβ1-4(Neu5Acα2-3)Galβ1-4Glcβ | NB | NB | NB |
| 40 | Fucα1-2Galβ1-3GalNAcβ1-4(Neu5Acα2-3)Galβ1-4Glcβ | NB | NB | NB |
| α2-6 sialosides | ||||
| 41 | Neu5Acα2-6Galβ1-4[6OSO3]GlcNAcβ | NB | NB | NB |
| 42 | Neu5Acα2-6Galβ1-4GlcNAcβ1-2Manα1-3(Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ | NB | NB | NB |
| 43 | Neu5Acα2-6Galβ1-4GlcNAcβ1-2Manα1-3(Neu5Acα2-6Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ | NB | NB | NB |
| 44 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-2Manα1-3[Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-2Manα1-6]Manβ1-4GlcNAcβ1-4GlcNAcβ | NB | NB | NB |
| 45 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-2Manα1-3[Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-2Manα1-6]-Manβ1-4GlcNAcβ1-4GlcNAcβ | NB | + | NB |
| 46 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3[Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-6]GalNAca | NB | NB | NB |
| 47 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3[Neu5Acα2-6Galβ1-4GlcNAcβ1-6]GalNAca | NB | NB | NB |
| 48 | Neu5Acα2-6GalNAcα | NB | NB | NB |
| 49 | Neu5Acα2-6Galβ | NB | NB | NB |
| 50 | Neu5Acα2-6Galβ1-4Glcβ | NB | NB | NB |
| 51 | Neu5Acα2-6Galβ1-4Glcβ | NB | NB | NB |
| 52 | Neu5Acα2-6Galβ1-4GlcNAcβ | NB | NB | NB |
| 53 | Neu5Acα2-6Galβ1-4GlcNAcβ | NB | NB | NB |
| 54 | Neu5Acα2-6GalNAcβ1-4GlcNAcβ | NB | NB | NB |
| 55 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3GalNAcα | NB | NB | NB |
| 56 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ | NB | NB | NB |
| 57 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3GalNAcα | NB | NB | NB |
| 58 | Neu5Aca2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ | NB | NB | NB |
| 59 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ1-4(Fucα1-3)GlcNAcβ | NB | NB | NB |
| 60 | Galβ1-3(Neu5Acα2-6)GlcNAcβ1-4Galβ1-4Glcβ-Sp10 | NB | NB | NB |
| 61 | Neu5Acα2-6[Galβ1-3]GalNAca | NB | NB | NB |
| 62 | Neu5Acα2-6Galβ1-4GlcNAcβ1-6[Galβ1-3]GalNAca | NB | NB | NB |
| 63 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-6[Galβ1-3]GalNAca | NB | NB | NB |
| Mixed α2-3 and α2-6 biantennaries | ||||
| 64 | Neu5Acα2-3Galβ1-4GlcNAcβ1-2Manα1-3(Neu5Acα2-6Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ | NB | NB | NB |
| 65 | Neu5Acα2-6Galβ1-4GlcNAcβ1-2Manα1-3(Neu5Acα2-3Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ | +++ | +++ | +++ |
| 66 | Neu5Acα2-3Galβ1-3(Neu5Acα2-6)GalNAcα | +++ | +++ | +++ |
| 67 | Neu5Acα2-3(Neu5Acα2-6)GalNAcα | NB | NB | NB |
| N-Glycolylneuraminic acid glycans | ||||
| 68 | Neu5Gcα | NB | NB | NB |
| 69 | Neu5Gcα2-3Galβ1-3(Fucα1-4)GlcNAcβ | NB | NB | NB |
| 70 | Neu5Gcα2-3Galβ1-3GlcNAcβ | NB | NB | NB |
| 71 | Neu5Gcα2-3Galβ1-4(Fucα1-3)GlcNAcβ | ++ | +++ | +++ |
| 72 | Neu5Gcα2-3Galβ1-4GlcNAcβ | NB | NB | NB |
| 73 | Neu5Gcα2-6GalNAcα | NB | NB | NB |
| 74 | Neu5Gcα2-6Galβ1-4GlcNAcβ | NB | NB | NB |
| α2-8-linked sialosides | ||||
| 75 | Neu5Acα2-8Neu5Acα | NB | NB | NB |
| 76 | Neu5Acα2-8Neu5Acα2-8Neu5Acα | NB | NB | NB |
| 77 | Neu5Acα2-8Neu5Acα2-3(GalNAcβ1-4)Galβ1-4Glcβ | NB | NB | NB |
| 78 | Neu5Acα2-8Neu5Acα2-3Galβ1-4Glcβ | NB | NB | NB |
| 79 | Neu5Acα2-8Neu5Acα2-8Neu5Acα2-3(GalNAcβ1-4)Galβ1-4Glcβ | NB | NB | NB |
| 80 | Neu5Acα2-8Neu5Acα2-8Neu5Acα2-3Galβ1-4Glcβ | NB | NB | NB |
| 81 | Neu5Acα2-8Neu5Acβ-Sp17 | NB | NB | NB |
| 82 | Neu5Acα2-8Neu5Acα2-8Neu5Acβ | NB | NB | NB |
| β2-6-linked and 9-O-acetylated sialic acids | ||||
| 83 | Neu5Acβ2-6GalNAcα | NB | NB | NB |
| 84 | Neu5Acβ2-6Galβ1-4GlcNAcβ | NB | NB | NB |
| 85 | Neu5Gcβ2-6Galβ1-4GlcNAc | NB | NB | NB |
| 86 | Galβ1-3(Neu5Acβ2-6)GalNAcα | NB | NB | NB |
| 87 | [9NAc]Neu5Acα | NB | NB | NB |
| 88 | [9NAc]Neu5Acα2-6Galβ1-4GlcNAcβ | NB | NB | NB |
| Asialoglycans | ||||
| 89 | Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ | NB | NB | NB |
| 90 | Galβ1-3GlcNAcβ1-3Galβ1-3GlcNAcβ | NB | NB | NB |
| 91 | Galβ1-4GlcNAcβ1-2Manα1-3[Galβ1-4GlcNAcβ1-2Manα1-6]Manβ1-4GlcNAcβ1-4GlcNAcβ | NB | NB | NB |
| 92 | GalNAcα1-3(Fucα1-2)Galβ1-3GlcNAcβ | NB | NB | NB |
| 93 | GalNAcα1-3(Fucα1-2)Galβ1-4GlcNAcβ | NB | NB | NB |
| 94 | Galα1-3(Fucα1-2)Galβ1-3GlcNAcβ | NB | NB | NB |
| 95 | Galα1-3(Fucα1-2)Galβ1-4(Fucα1-3)GlcNAcβ | NB | NB | NB |
| 96 | Galβ1-3GalNAcα | NB | NB | NB |
a
Neu5Ac, sialic acid; Neu5Gc, N-glycolylneuraminic acid; OSO3, sulfate; Gal, galactose; Fuc, fucose; Glc, d-glucose; GlcNAc, N-acetyl-d-glucosamine; GalNAc, N-acetyl-d-galactosamine; Man, d-mannose; 9NAc, 9-O-acetyl.
b
Significant binding of samples to glycans was estimated qualitatively based on the relative strength of the signal for the data shown in Fig. 3. Fluorescence intensity is indicated as follows: +++, >3,000; ++, 2,000 to 2,999; +, 1,000 to 1,999; NB, <1,000 (no binding observed).