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. 2016 May 27;90(12):5770–5784. doi: 10.1128/JVI.00180-16

TABLE 2.

Glycan microarray for H5Nx HAs

Glycan no. by category Structurea Bindingb
A/northern
pintail/
Washington/
40964/14
A/Sichuan/
26221/14
A/gyrfalcon/
Washington/
41088-6/14
Sialic acid
    1 Neu5Acα NB NB NB
    2 Neu5Acα NB NB NB
    3 Neu5Acβ NB NB NB
α2-3 sialosides
    4 Neu5Acα2-3(6-O-Su)Galβ1-4GlcNAcβ +++ +++ +++
    5 Neu5Acα2-3Galβ1-3[6OSO3]GalNAcα +++ +++ +++
    6 Neu5Acα2-3Galβ1-4[6OSO3]GlcNAcβ +++ +++ +++
    7 Neu5Acα2-3Galβ1-4(Fucα1-3)[6OSO3]GlcNAcβ-propyl-NH2 +++ +++ +++
    8 Neu5Acα2-3Galβ1-3[6OSO3]GlcNAc-propyl-NH2 +++ +++ +++
    9 Neu5Acα2-3Galβ1-3(Neu5Acα2-3Galβ1-4)GlcNAcβ +++ +++ +++
    10 Neu5Acα2-3Galβ1-3(Neu5Acα2-3Galβ1-4GlcNAcβ1-6)GalNAcα +++ +++ +++
    11 Neu5Acα2-3Galβ1-4GlcNAcβ1-2Manα1-3(Neu5Acα2-3Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ +++ +++ +++
    12 Neu5Acα(2-3)-Galβ(1-4)-GlcNAcβ(1-3)-Galβ(1-4)-GlcNAcβ(1-2)-Manα(1-3)-[Neu5Acα(2-3)-Galβ(1-4)-GlcNAcβ(1-3)-Galβ(1-4)-GlcNAcβ(1-2)-Manα(1-6)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ +++ +++ +++
    13 Neu5Acα2-3Galβ +++ +++ ++
    14 Neu5Acα2-3Galβ1-3GalNAcα +++ +++ +++
    15 Neu5Acα2-3Galβ1-3GlcNAcβ +++ +++ ++
    16 Neu5Acα2-3Galβ1-3GlcNAcβ +++ +++ +++
    17 Neu5Acα2-3Galβ1-4Glcβ +++ +++ +++
    18 Neu5Acα2-3Galβ1-4Glcβ +++ +++ +++
    19 Neu5Acα2-3Galβ1-4GlcNAcβ +++ +++ +++
    20 Neu5Acα2-3Galβ1-4GlcNAcβ +++ +++ +++
    21 Neu5Acα2-3GalNAcβ1-4GlcNAcβ NB NB ++
    22 Neu5Acα2-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ +++ +++ +++
    23 Neu5Aca2-3Galβ1-3GlcNAcβ1-3Galβ1-4GlcNAcβ +++ +++ +++
    24 Neu5Acα2-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ +++ +++ +++
    25 Neu5Acα2-3Galβ1-4GlcNAcβ1-3Galβ1-3GlcNAcβ +++ +++ +++
    26 Neu5Acα2-3Galβ1-3GalNAcα +++ +++ +++
    27 Galβ1-3(Neu5Acα2-3Galβ1-4(Fucα1-3)GlcNAcβ1-6)GalNAcα NB + NB
    28 Neu5Acα2-3Galβ1-3(Fucα1-4)GlcNAcβ +++ +++ +++
    29 Neu5Acα2-3Galβ1-4(Fucα1-3)GlcNAcβ +++ +++ +++
    30 Neu5Acα2-3Galβ1-4(Fucα1-3)GlcNAcβ +++ +++ +++
    31 Neu5Acα2-3Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ +++ +++ +++
    32 Neu5Acα2-3Galβ1-3[Fucα1-4]GlcNAcβ1-3Galβ1-4[Fucα1-3]GlcNAcβ +++ +++ +++
    33 Neu5Acα2-3Galβ1-3[Fucα1-3]GlcNAcβ1-3Galβ1-4[Fucα1-3]GlcNAcβ +++ +++ +++
    34 Neu5Acα2-3Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ1-4(Fucα1-3)GlcNAcβ +++ +++ +++
    35 Neu5Acα2-3(GalNAcβ1-4)Galβ1-4GlcNAcβ NB NB NB
    36 Neu5Acα2-3(GalNAcβ1-4)Galβ1-4GlcNAcβ NB NB NB
    37 Neu5Acα2-3(GalNAcβ1-4)Galβ1-4Glcβ NB NB NB
    38 Galβ1-3GalNAcβ1-4(Neu5Acα2-3)Galβ1-4Glcβ NB NB NB
    39 Fucα1-2Galβ1-3GalNAcβ1-4(Neu5Acα2-3)Galβ1-4Glcβ NB NB NB
    40 Fucα1-2Galβ1-3GalNAcβ1-4(Neu5Acα2-3)Galβ1-4Glcβ NB NB NB
α2-6 sialosides
    41 Neu5Acα2-6Galβ1-4[6OSO3]GlcNAcβ NB NB NB
    42 Neu5Acα2-6Galβ1-4GlcNAcβ1-2Manα1-3(Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ NB NB NB
    43 Neu5Acα2-6Galβ1-4GlcNAcβ1-2Manα1-3(Neu5Acα2-6Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ NB NB NB
    44 Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-2Manα1-3[Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-2Manα1-6]Manβ1-4GlcNAcβ1-4GlcNAcβ NB NB NB
    45 Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-2Manα1-3[Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-2Manα1-6]-Manβ1-4GlcNAcβ1-4GlcNAcβ NB + NB
    46 Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3[Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-6]GalNAca NB NB NB
    47 Neu5Acα2-6Galβ1-4GlcNAcβ1-3[Neu5Acα2-6Galβ1-4GlcNAcβ1-6]GalNAca NB NB NB
    48 Neu5Acα2-6GalNAcα NB NB NB
    49 Neu5Acα2-6Galβ NB NB NB
    50 Neu5Acα2-6Galβ1-4Glcβ NB NB NB
    51 Neu5Acα2-6Galβ1-4Glcβ NB NB NB
    52 Neu5Acα2-6Galβ1-4GlcNAcβ NB NB NB
    53 Neu5Acα2-6Galβ1-4GlcNAcβ NB NB NB
    54 Neu5Acα2-6GalNAcβ1-4GlcNAcβ NB NB NB
    55 Neu5Acα2-6Galβ1-4GlcNAcβ1-3GalNAcα NB NB NB
    56 Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ NB NB NB
    57 Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3GalNAcα NB NB NB
    58 Neu5Aca2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ NB NB NB
    59 Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ1-4(Fucα1-3)GlcNAcβ NB NB NB
    60 Galβ1-3(Neu5Acα2-6)GlcNAcβ1-4Galβ1-4Glcβ-Sp10 NB NB NB
    61 Neu5Acα2-6[Galβ1-3]GalNAca NB NB NB
    62 Neu5Acα2-6Galβ1-4GlcNAcβ1-6[Galβ1-3]GalNAca NB NB NB
    63 Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-6[Galβ1-3]GalNAca NB NB NB
Mixed α2-3 and α2-6 biantennaries
    64 Neu5Acα2-3Galβ1-4GlcNAcβ1-2Manα1-3(Neu5Acα2-6Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ NB NB NB
    65 Neu5Acα2-6Galβ1-4GlcNAcβ1-2Manα1-3(Neu5Acα2-3Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ +++ +++ +++
    66 Neu5Acα2-3Galβ1-3(Neu5Acα2-6)GalNAcα +++ +++ +++
    67 Neu5Acα2-3(Neu5Acα2-6)GalNAcα NB NB NB
N-Glycolylneuraminic acid glycans
    68 Neu5Gcα NB NB NB
    69 Neu5Gcα2-3Galβ1-3(Fucα1-4)GlcNAcβ NB NB NB
    70 Neu5Gcα2-3Galβ1-3GlcNAcβ NB NB NB
    71 Neu5Gcα2-3Galβ1-4(Fucα1-3)GlcNAcβ ++ +++ +++
    72 Neu5Gcα2-3Galβ1-4GlcNAcβ NB NB NB
    73 Neu5Gcα2-6GalNAcα NB NB NB
    74 Neu5Gcα2-6Galβ1-4GlcNAcβ NB NB NB
α2-8-linked sialosides
    75 Neu5Acα2-8Neu5Acα NB NB NB
    76 Neu5Acα2-8Neu5Acα2-8Neu5Acα NB NB NB
    77 Neu5Acα2-8Neu5Acα2-3(GalNAcβ1-4)Galβ1-4Glcβ NB NB NB
    78 Neu5Acα2-8Neu5Acα2-3Galβ1-4Glcβ NB NB NB
    79 Neu5Acα2-8Neu5Acα2-8Neu5Acα2-3(GalNAcβ1-4)Galβ1-4Glcβ NB NB NB
    80 Neu5Acα2-8Neu5Acα2-8Neu5Acα2-3Galβ1-4Glcβ NB NB NB
    81 Neu5Acα2-8Neu5Acβ-Sp17 NB NB NB
    82 Neu5Acα2-8Neu5Acα2-8Neu5Acβ NB NB NB
β2-6-linked and 9-O-acetylated sialic acids
    83 Neu5Acβ2-6GalNAcα NB NB NB
    84 Neu5Acβ2-6Galβ1-4GlcNAcβ NB NB NB
    85 Neu5Gcβ2-6Galβ1-4GlcNAc NB NB NB
    86 Galβ1-3(Neu5Acβ2-6)GalNAcα NB NB NB
    87 [9NAc]Neu5Acα NB NB NB
    88 [9NAc]Neu5Acα2-6Galβ1-4GlcNAcβ NB NB NB
Asialoglycans
    89 Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ NB NB NB
    90 Galβ1-3GlcNAcβ1-3Galβ1-3GlcNAcβ NB NB NB
    91 Galβ1-4GlcNAcβ1-2Manα1-3[Galβ1-4GlcNAcβ1-2Manα1-6]Manβ1-4GlcNAcβ1-4GlcNAcβ NB NB NB
    92 GalNAcα1-3(Fucα1-2)Galβ1-3GlcNAcβ NB NB NB
    93 GalNAcα1-3(Fucα1-2)Galβ1-4GlcNAcβ NB NB NB
    94 Galα1-3(Fucα1-2)Galβ1-3GlcNAcβ NB NB NB
    95 Galα1-3(Fucα1-2)Galβ1-4(Fucα1-3)GlcNAcβ NB NB NB
    96 Galβ1-3GalNAcα NB NB NB
a

Neu5Ac, sialic acid; Neu5Gc, N-glycolylneuraminic acid; OSO3, sulfate; Gal, galactose; Fuc, fucose; Glc, d-glucose; GlcNAc, N-acetyl-d-glucosamine; GalNAc, N-acetyl-d-galactosamine; Man, d-mannose; 9NAc, 9-O-acetyl.

b

Significant binding of samples to glycans was estimated qualitatively based on the relative strength of the signal for the data shown in Fig. 3. Fluorescence intensity is indicated as follows: +++, >3,000; ++, 2,000 to 2,999; +, 1,000 to 1,999; NB, <1,000 (no binding observed).