. 2016 May 27;90(12):5770–5784. doi: 10.1128/JVI.00180-16

TABLE 3.

Kinetic results for glycan binding to WT and mutant H5Nx recHAs^a

RecHA	Glycan	Apparent K_D (μM)	k_on (ms⁻¹)	k_obs ± SE (10⁻² s⁻¹)	k_off ± SE (10⁻² s⁻¹)
Gyr14_WT	3SLNLN-b	1.26	75,286	50.71 ± 2.61	9.52 ± 0.65
Gyr14_WT	6SLNLN-b	NB	NB	NB	NB
Gyr14_136	3SLNLN-b	1.32	84,740	57.88 ± 3.24	11.18 ± 0.71
Gyr14_136	6SLNLN-b	NB	NB	NB	NB
Gyr14_141	3SLNLN-b	1.10	81,281	53.75 ± 2.85	8.97 ± 0.60
Gyr14_141	6SLNLN-b	NB	NB	NB	NB
Gyr14_136_141	3SLNLN-b	1.43	86,241	59.88 ± 3.49	12.36 ± 0.79
Gyr14_136_141	6SLNLN-b	NB	NB	NB	NB
pin14_WT	3SLNLN-b	1.88	94,675	69.39 ± 4.16	17.59 ± 0.87
pin14_WT	6SLNLN-b	NB	NB	NB	NB
pin14_141	3SLNLN-b	0.30	85,474	49.34 ± 2.40	2.58 ± 0.21
pin14_141	6SLNLN-b	NB	NB	NB	NB
Sich14	3SLNLN-b	0.93	81,637	52.55 ± 2.84	7.63 ± 0.51
Sich14	6SLNLN-b	NB	NB	NB	NB
SwH3_WT	3SLNLN-b	NB	NB	NB	NB
SwH3_WT	6SLNLN-b	141.2	2,040	30.23 ± 1.95	28.81 ± 1.78

K_D, equilibrium dissociation constant; k_on, k_obs, and k_off, association, observed, and dissociation rates, respectively; NB, no detectable binding.