TABLE 3.
RecHA | Glycan | Apparent KD (μM) | kon (ms−1) | kobs ± SE (10−2 s−1) | koff ± SE (10−2 s−1) |
---|---|---|---|---|---|
Gyr14_WT | 3SLNLN-b | 1.26 | 75,286 | 50.71 ± 2.61 | 9.52 ± 0.65 |
6SLNLN-b | NB | NB | NB | NB | |
Gyr14_136 | 3SLNLN-b | 1.32 | 84,740 | 57.88 ± 3.24 | 11.18 ± 0.71 |
6SLNLN-b | NB | NB | NB | NB | |
Gyr14_141 | 3SLNLN-b | 1.10 | 81,281 | 53.75 ± 2.85 | 8.97 ± 0.60 |
6SLNLN-b | NB | NB | NB | NB | |
Gyr14_136_141 | 3SLNLN-b | 1.43 | 86,241 | 59.88 ± 3.49 | 12.36 ± 0.79 |
6SLNLN-b | NB | NB | NB | NB | |
pin14_WT | 3SLNLN-b | 1.88 | 94,675 | 69.39 ± 4.16 | 17.59 ± 0.87 |
6SLNLN-b | NB | NB | NB | NB | |
pin14_141 | 3SLNLN-b | 0.30 | 85,474 | 49.34 ± 2.40 | 2.58 ± 0.21 |
6SLNLN-b | NB | NB | NB | NB | |
Sich14 | 3SLNLN-b | 0.93 | 81,637 | 52.55 ± 2.84 | 7.63 ± 0.51 |
6SLNLN-b | NB | NB | NB | NB | |
SwH3_WT | 3SLNLN-b | NB | NB | NB | NB |
6SLNLN-b | 141.2 | 2,040 | 30.23 ± 1.95 | 28.81 ± 1.78 |
KD, equilibrium dissociation constant; kon, kobs, and koff, association, observed, and dissociation rates, respectively; NB, no detectable binding.