FIG 2.

VP24 inhibits the activation of IFN-β promoter through IRF3 elements. (A to D) VP24-Flag plasmid was cotransfected into HEK 293T cells with a NF-κB reporter plasmid (200 ng) (A and C) or an IRF3 reporter plasmid (200 ng) (B and D) with cGAS (15 ng) and a minimal amount of STING (2.5 ng) plasmid or a large amount of STING plasmid (200 ng), respectively. Cells were harvested 24 h posttransfection and subjected to the DLR assay. The data represent results from one of the triplicate experiments.