FIG 4.

VP24 blocks the phosphorylation and dimerization of IRF3. (A) HFF-shNC/shVP24 cells were infected with WT HSV at an MOI of 5 for 2 h before transfection with ISD. Western blot analysis was performed 16 h posttransfection to detect IRF3 phosphorylation. (B) HFF-shNC/shVP24 cells were mock infected or infected with WT HSV at an MOI of 5 for 2 h before transfection with ISD. Cells were harvested at 18 hpi, and native PAGE assays were performed to detect IRF3 dimerization. The data represent results from one of the triplicate experiments.