FIG 5.

VP24 prevents the interaction between endogenous TBK1 and IRF3 during HSV-1 infection by interacting with IRF3. (A) HFF-shNC/shVP24 cells were infected with WT HSV at an MOI of 5 for 2 h before transfection with ISD. Cells were harvested at 18 hpi, and the extracts were analyzed by immunoprecipitation using anti-TBK1 PAb (IP:TBK1), and Western blotting with anti-TBK1, anti-IRF3, and anti-VP24. The data represent results from one of the triplicate experiments. (B) HEK 293T cells were transfected with VP24-HA plasmid and harvested at 36 h posttransfection, and the extracts were analyzed by immunoprecipitation using IRF3 PAb (IP:IRF3) and WB with anti-HA and anti-IRF3. The data represent results from one of the triplicate experiments.