FIG 2.

16E6 does not alter E7-mediated pAKT attenuation. (A) Retrovirally transduced keratinocytes with 16E7 or 16E6 were plated and harvested as described for Fig. 1A. Statistics were calculated from three independent experiments. Bar represents the removal of a redundant time point. (B) Retrovirally transduced keratinocytes with 16E7 or 16E6 were plated and harvested as described for Fig. 1A, except that cells were maintained in KSFM. Statistics were calculated from three independent experiments. (C) Retrovirally transduced keratinocytes with 16E7 or 16E6 were plated and subsequently harvested 3 days later in 1% SDS. Statistics were calculated from three independent experiments. (D) Retrovirally transduced keratinocytes with 16E7 or 16E6 were plated and harvested as described for panel B. Statistics were calculated from three independent experiments. (E) Retrovirally transduced keratinocytes with 16E7 or 16E7/16E6 were plated and harvested as described for Fig. 1A. Statistics were calculated from three independent experiments. (*, P < 0.05; **, P < 0.01; ***, P < 0.001). (F) Retrovirally transduced keratinocytes with 16E7 or 16E7/16E6 were plated and harvested as described for Fig. 2B. Statistics were calculated from four independent experiments. (*, P < 0.05; **, P < 0.01; ***, P < 0.001).