FIG 7.

HPV-16 E7 alters cap- versus IRES-dependent translation. NIKS that were retrovirally transduced and selected to express either vector or 16E7 were transfected with a bicistronic-reporter plasmid (shown at the top) and 48 h later were starved for 12 h. Following starvation, transduced NIKS were assayed for firefly and Renilla luciferase activity. Values were normalized to vector-transduced cells. Statistics were calculated from three independent experiments each performed in duplicate (**, P < 0.005).