FIG 2.
Western blot detection of PrPSc from HY TME- and 139H-coinfected hamsters. Brain homogenates from hamsters infected with either the hyper (HY) 139H or drowsy (DY) TME strain at terminal disease were digested with proteinase K, and the unglycosylated PrPSc polypeptide migrates at 21 kDa for the HY and 139H strains and at 19 kDa for the DY strain. In animals first infected with 139H and then superinfected with HY at 25, 50, or 75 days after 139H infection, PrPSc was detected in the brains of all animals. Due to the similar PrPSc Western blot properties for HY and 139H, it is not possible to determine which strain caused disease. The 19- and 21-kDa unglycosylated PrPSc polypeptides are indicated at the left. n.a., not applicable.