FIG 5.
(A) Electron micrograph of an S. islandicus ΔC1C2 culture infected with SMV1 (high MOI) at 22 hpi. Scale bar, 1 μm. (B) Time course analysis of the percentage of live (light gray) and dead (dark gray) cells in an S. islandicus ΔC1C2 culture infected with SMV1 (MOI ≈ 3) compared to an uninfected control. (C) Flow cytometry analysis of live/dead staining of S. islandicus ΔC1C2 before and after infection with SMV1 (MOI of 3) at 22 hpi. S. solfataricus 5E6 cells infected with SIRV2 (MOI of 5) were used as a positive control for DNA degradation and cell lysis.