FIG 6.

Rescue of capsid assembly of CTD-deleted HBc by WT HBc in RRL. WT or C149 HBc was translated alone (A, top or middle, respectively; B, lane 1 or lane 2, respectively), or WT HBc and C149 HBc were translated together (A, bottom; B, lanes 3) in RRL. The capsids were induced to assemble using CIAP treatment, and the assembled capsids were resolved by sucrose gradient centrifugation as described for Fig. 4. The translation reaction mixtures (A, lane 1, 0.5 μl), the assembly reaction mixtures (i.e., the input to the gradient; CIAP) (A, lane 2, 0.5 μl; B, lanes 1 to 3, 5 μl), and the indicated sucrose fractions (A, lanes 3 to 15, 10 μl; B, lanes 4 to 11, 50 μl) were resolved by agarose gel electrophoresis (A) or SDS-PAGE (B) and detected by autoradiography. C, WT HBc subunits; C149, C-terminally truncated HBc protein (terminated at position 149); Ca, capsids; *, novel band appearing only in the mixed translation and migrating between WT and C149 HBc subunits. The direction of centrifugation is indicated by the arrow in panel A.