FIG 3.

Detection of virus release for wild-type virus and FL mutant viruses. (A) Representative data for Gag/CA release and processing in mock-, WT-, and R86A-transfected QT6 cells. CA-related proteins were immunoprecipitated from cell lysate (L) and medium (M) samples, as described in Materials and Methods. (B) Particle release as quantified from measurements of ³⁵S-labeled CA in supernatants of transfected cells, normalized for Gag expression, as described in Materials and Methods. All particle release experiments were performed in triplicate.