TABLE 1.

Gag release and virus infectivity phenotypes of CA flexible-loop mutant viruses

WT residue	Mutant residue	Gag/CA release (mean ± SD)a,b	Rate of spread (mean ± SD)b,c
All		1.44 ± 0.46	0.80 ± 0.17
R86	A	1.38 ± 0.42	0.62 ± 0.07
	D	1.28 ± 0.17	≤0.05***
	K	1.84 ± 0.32	0.73 ± 0.14
D87	A	0.83 ± 0.73	0.08 ± 0.09***
	R	0.00 ± 0.01**	≤0.05***
	E	1.92 ± 0.69	0.28 ± 0.02***
	N	NDd	0.14 ± 0.08***
R89	A	1.19 ± 0.75	0.57 ± 0.07*
	D	1.14 ± 0.60	≤0.05***
	K	1.01 ± 0.02	0.58 ± 0.25*
Q95	A	1.07 ± 0.31	0.89 ± 0.06
	E	0.46 ± 0.44	0.78 ± 0.09
E99	A	1.25 ± 0.48	0.41 ± 0.04**
	K	0.73 ± 0.93	≤0.05***
	D	0.89 ± 0.81	0.70 ± 0.02
	Q	ND	0.19 ± 0.03***
N104	A	0.58 ± 0.43	0.59 ± 0.07*
	R	ND	≤0.05***
	D	ND	0.63 ± 0.001*
K107	A	1.02 ± 0.85	≤0.05***
	D	0.33 ± 0.46	≤0.05***
	R	1.75 ± 0.45	0.30 ± 0.04***

^aParticle release was quantified from measurements of ³⁵S-labeled CA in supernatants of transfected cells, normalized for Gag expression, as described in Materials and Methods.

^bStatistical analyses are described in the legend to Fig. 2C. ***, P < 0.001; **, P = 0.001 to 0.01; *, P = 0.01 to 0.05.

^cRates of virus spread were calculated from values for the percentage of GFP-expressing DF1 cells during the exponential phase of virus spread at 37°C and are expressed as Δln(percent green cells) per day (60).

^dND, not done.