FIG 2.

Loss of hCAR glycosylation has no influence on CVB3 attachment. (A) Virus binding to CHO-K1 cells transfected with hCAR glycosylation variants was determined as described in Materials and Methods. CHO-K1 cells exposed to transfection reagent without plasmid DNA (mock) served as a negative control. (B) Different glycosylated CAR variants were expressed as soluble receptors fused to human IgG-Fc, and equal amounts were bound to protein G-coated magnetic beads. Soluble Fc was used as negative control. Bead-coupled soluble receptor proteins were incubated with ³⁵S-labeled CVB3 (10,000 cpm) for 1 h at 4°C. After washing, bound virus was measured by scintillation counting.