TABLE 1.

All seven animals vaccinated with m3KOΔnef are resistant to intrarectal challenge with SIVmac239^a

Challenge virus and animal	Viral load (ceq/ml plasma)
	Day 0	Day 7	Day 14	Day 21
ΔNef
cy0348	<50	<50	<50	<50
cy0379	82,500	21,500	29,000	102,000
cy0381	681	178	358	273
cy0382	<50	<50	<50	<50
cy0384	<50	<50	<50	<50
cy0383	<50	<50	<50	54
cy0385	4,730	231	1,330	7,660
Full-length Nef
cy0348	<50	<50	<50	<50
cy0379	<50	<50	<50	<50
cy0381	<50	<50	<50	<50
cy0382	<50	<50	<50	<50
cy0384	<50	<50	<50	<50
cy0383	<50	<50	<50	<50
cy0385	<50	<50	<50	<50

^aThe time points listed indicate the number of days after intrarectal challenge. nef qRT-PCR was performed on plasma samples after SIVmac239 challenge. Viral loads for the 182-bp nef deletion and full-length nef were measured. A viral load of <50 virus copy equivalents (ceq) per ml of blood plasma represents the limit of detection.