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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: DNA Repair (Amst). 2016 Mar 25;41:8–15. doi: 10.1016/j.dnarep.2016.03.008

Figure 2. ZK742.2 is involved in the repair of UV-induced DNA damage.

Figure 2

(A) The percentage of larval stages 48 h after UV-B treatment administered at the L1 larval stage. A minimum of 500 animals were used for each UV-B-dose, and each treatment was conducted in triplicate. Larval stages were determined by flow cytometry. Error bars represent SD, statistical analysis is a two-tailed t-test comparing treatment groups to wildtype, *=p<0.05, **=p<0.01, ***=p<0.001. (B) Slot-blot DNA repair assay using α-CPD antibody. Wildtype, ZK742.2(tm6311) and csb-1 animals were treated with 60 mJ/cm2 UV-B at L1 larval stage and CPD lesions were measured by antibody staining in slot blots of worm extracts directly after treatment and 24 h post-UV. The average relative CPD load were calculated comparing 24 h to 0 h and the values shown are the average of two independent experiments.