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. Author manuscript; available in PMC: 2016 Oct 21.
Published in final edited form as: Nature. 2016 Apr 13;532(7599):375–379. doi: 10.1038/nature17407

Figure 4. Magnesium transport phase-dependently affects cellular energy balance.

Figure 4

Cells were treated with CHA or vehicle during the second half of the day (black bars) or night (blue bars) to test acute effects on magnesium (a) and ATP (b) levels in both cell types. Incorporation of puromycin was analysed by western blot (c) then quantified to give relative translation rates at the indicated times (d). Mean±SEM are shown, n=3, t-test significance reported (n.s., p>0.18). e, Simplified model for a feedback mechanism between circadian [Mg2+]i rhythms and the clockwork to temporally regulate global cellular metabolism; orange arrow represents extracellular stimuli.