Figure 2.

Standardized extract of Ginkgo biloba (EGb761)-dependent changes in aldosterone, cortisol, testosterone, and 17β-estradiol concentrations in H295R cells. Adherent cells were exposed to EGb761 (10 and 100 μg/mL) for 24 hours. Hormone concentrations were measured by competitive enzyme-linked immunospecific assay following the manufacturer’s recommendations. The results are expressed as the mean±standard deviation of three separate experiments for each group. Values are significantly different from the control: ^**p<0.01. Hormone basal level: aldosterone (136.16±6.94 pg/mL); cortisol (2014.62±149.32 pg/mL); testosterone (182.25±25.79 pg/mL); 17β-estradiol (181.48±27.46 pg/mL).