Skip to main content
. 2016 May 9;31:e2016010. doi: 10.5620/eht.e2016010

Table 1.

Conditions for quantitative reverse-transcriptase polymerase chain reaction

Gene Amplicon size (bp) Annealing temperature (°C) Forward primer (5’→3’) Reverse primer (5’→3’)
β-actin 100 64 CACTCTTCCAGCCTTCCTTCC AGGTCTTTGCGGATGTCCAC
CYP11A 137 62 GAGATGGCACGCAACCTGAAG CTTAGTGTCTCCTTGATGCTGGC
CYP11B1 541 62 GGCAGAGGCAGAGATGCTG TCTTGGGTTAGTGTCTCCACCTG
CYP11B2 146 62 TCCAGGTGTGTTCAGTAGTTCC GAAGCCATCTCTGAGGTCTGTG
CYP17A1 134 64 AGCCGCACACCAACTATCAG TCACCGATGCTGGAGTCAAC
CYP19 128 64 AGGTGCTATTGGTCATCTGCTC TGGTGGAATCGGGTCTTTATGG
CYP21A2 108 64 CGTGGTGCTGACCCGACTG GGCTGCATCTTGAGGATGACAC
3β-HSD2 95 60 TGCCAGTCTTCATCTACACCAG TTCCAGAGGCTCTTCTTCGTG
17β-HSD1 136 64 CTCCCTCTGACCAGCAACC TGTGTCTCCCACGCAATCTC
17β-HSD4 121 62 TGCGGGATCACGGATGACTC GCCACCATTCTCCTCACAACTC