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| Reagent | Amount to add (for 1 L) | Final concentration |
|---|---|---|
| Agar | 17.0 g | 1.7% (w/v) |
| NaCl | 2.9 g | 50 mм |
| Peptone | 2.5 g | 0.25% (w/v) |
| CaCl2 (1 м) | 1 mL | 1 mм |
| Cholesterol (5 mg/mL) | 1 mL | 5 μg/mL |
| KH2PO4 (1 м) | 25 mL | 25 mм |
| MgSO4 (1 м) | 1 mL | 1 mм |
Mix the first three reagents and autoclave. After the mixture is cool, add the last four reagents.
